SpeedsPath Glossary of Terms

Formalin-Fixed Paraffin-Embedded (FFPE)

FFPE (Formalin-Fixed Paraffin-Embedded) refers to a standard histopathology sample preparation method where biological tissues are first preserved using formalin fixation and then embedded into paraffin wax. This technique stabilizes tissue morphology and cellular structures for long-term storage and microscopic examination. In pathology Lab Management Software, FFPE records are essential for managing slides, blocks, archival materials, traceability, and downstream processes like molecular testing.

What Is FFPE?

FFPE stands for Formalin-Fixed Paraffin-Embedded, a standard method for preparing tissue samples in histopathology. Tissues are fixed in formalin to preserve structure, then embedded in paraffin wax for sectioning into thin slides. This allows for microscopic examination and various diagnostic tests.

Why FFPE Matters in Pathology Labs?

FFPE is crucial for preserving tissue samples for long-term use in diagnosis and research.

Key reasons include:

• Preserves tissue samples using formalin fixation and paraffin embedding for long-term use.
• Enables creation and tracking of tissue blocks and slides within LIMS.
• Supports histology workflows such as microtomy, staining, and slide scanning.
• Facilitates barcode-based identification of blocks and slides for error-free tracking.
• Allows attachment of microscopic images and WSI (Whole Slide Images) to FFPE entries.
• Supports inventory management of archived FFPE blocks and slides.
• Provides linkage between specimen → block → slide → result in LIMS.
• Ensures chain-of-custody and audit trail for FFPE sample handling.
• Enables retrieval of stored FFPE blocks for IHC, molecular tests, NGS, PCR, etc.
• Helps maintain regulatory compliance with CAP/CLIA/FDA for sample processing and storage.

How FFPE Works in a Laboratory Information System (LIS)?

In a modern LIS like SpeedsPath, FFPE management is integrated into the workflow. The system tracks fixation, embedding, block creation, slide preparation, and storage. Barcodes ensure traceability, and digital records link to images and test results.

Benefits of Using LIS for FFPE Management

• Accurate tracking of blocks and slides
• Barcode integration for error reduction
• Inventory management for archives
• Compliance with regulatory standards
• Efficient retrieval and testing

SpeedsPath Makes FFPE Management Efficient

FFPE is fundamental to histopathology. With LIS automation, labs can manage samples effectively, ensuring quality and compliance.

See how SpeedsPath handles FFPE tracking and workflows.

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Related Terms

• Histopathology
• Tissue Block
• Slide
• Barcode
• LIMS

FAQs

1. What does FFPE mean in a pathology LIMS?
   It refers to the digital representation and tracking of tissue samples that have undergone formalin fixation and paraffin embedding.
2. Why is FFPE important for histopathology workflows?
   It preserves tissue morphology and allows creation of tissue blocks and slides used for diagnosis, IHC, and molecular testing.
3. Can LIMS track FFPE blocks and slides separately?
   Yes. LIMS typically assigns unique barcodes to each block and all associated slides.
4. Does LIMS support storing images of FFPE slides?
   Yes. Whole-slide images, microphotographs, and annotated images can be uploaded or linked.
5. How does FFPE data improve sample traceability?
   Each step—fixation, embedding, sectioning, staining—is logged, ensuring complete chain-of-custody.
6. Can FFPE samples be retrieved from archives through the software?
   Yes. LIMS includes archive location mapping (rack, box, position) for quick retrieval.
7. Does the system handle IHC or molecular testing on FFPE tissue?
   Most LIMS platforms allow linking FFPE blocks to IHC orders, molecular workflows, and test results.
8. How long can FFPE samples be stored?
   Hospitals often store FFPE blocks for years. LIMS helps track retention periods and expiry rules.
9. Can LIMS generate labels for FFPE blocks and slides?
   Yes. It supports barcode/QR code label printing to minimize identification errors.
10. Is FFPE information included in pathology reports?
    Yes. Reports may include block/slide IDs, staining details, and microscopic observations derived from FFPE samples.

Want to learn more? Explore our LIS Glossary or check out our expert blogs on Anatomic Pathology, Molecular Diagnostics, Cytology, and Lab Information Systems.